Melioidosis Reference Manual
Published in 2022.
Developed by Menzies School of Health Research, Darwin, Northern Territory, Australia.
Supported by American Society for Microbiology and U.S. Centers for Disease Control and Prevention.
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The full manual pdf can be accessed and downloaded HERE:
By parts:
Chapter 1: Overview of Melioidosis .......................................................................................... 2
Chapter 2: Clinical Presentations and Disease Progression .................................................... 10
Chapter 3: Handling in the Field and Personal Protective Equipment (PPE) .......................... 31
Chapter 4: B. pseudomallei Laboratory Biosafety ................................................................... 38
Chapter 5: Clinical Specimen Collection .................................................................................. 49
Chapter 6: Clinical Specimen Packaging and Transport .......................................................... 67
Chapter 7: Environmental Sampling for the Detection and Surveillance of B. pseudomallei . 79
Chapter 8: Specimen Processing and Diagnostic Testing Methods Overview ........................ 97
Chapter 9: Testing Workflows for the Identification of B. pseudomallei .............................. 118
Chapter 10: B. pseudomallei Bacterial Culture Detection ..................................................... 127
Chapter 11: B. pseudomallei Antibody Serology and Direct Antigen Detection Methods .... 160
Chapter 12: Polymerase Chain Reaction (PCR) Overview ..................................................... 183
Chapter 13: B. pseudomallei DNA extraction from Clinical and Environmental Specimens . 204
Chapter 14: Conventional Polymerase Chain Reaction (PCR) ............................................... 251
Chapter 15: Real-Time Polymerase Chain Reaction (PCR) Detection of B. pseudomallei ..... 277
Chapter 16: Molecular Methods for B. pseudomallei Bacterial Strain Typing ...................... 305
Chapter 17: B. pseudomallei Suspected Isolate Reporting, Storage and Data Management 347
Chapter 18: Melioidosis Surveillance and Filing a Clinical Case Report Form ....................... 356
Glossary ................................................................................................................................. 364
References ............................................................................................................................. 367
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