Detection of environmental B. pseudomallei indicates a potential risk for melioidosis, and is important for the development of a global risk map. Here, we describe a simple method to detect B. pseudomallei using direct soil culture in enrichment broth. Based on statistical considerations described in the accompanying review, a minimum of 100 sampling points is suggested. This method is currently used at the Mahidol-Oxford Tropical Medicine Research Unit Thailand, but variations are possible as described in the review and consensus guidelines.