From Mahidol-Oxford Tropical Medicine Research Unit (MORU), Bangkok, THailand

Burkholderia pseudomallei is a non-fermenting Gram-negative bacillus that is oxidase positive. The culture of B. pseudomallei from any specimen remains the gold standard for diagnosis of melioidosis. Samples of blood, urine and a throat swab should be obtained for culture from all patients, together with respiratory secretions, pus and wound swab where relevant. All specimens from patients with suspected melioidosis should be handled with the appropriate safety precautions. The organism grows well on most routine laboratory media but specimens from non-sterile sites can benefit from the use of selective media (such as Ashdown’s agar and selective broth), which permit the growth of B. pseudomallei and suppress other organisms.

Posters in English (used during the workshop at World Melioidosis Congress 8th, Cebu, Philippines, 2016) 

Posters in Thai (translated from the English version) 

The methods for isolation, identification and susceptibility testing of B. pseudomallei are detailed in the following sections (i.e. click "More.." for SOP)